ExperimentMic multiplexed FISH on cerebral cortex

multiplexed FISH4DNEXWI45RB4
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5 FOVs

   May 16th, 2022 at 12:31pm
Source Publication
Single-cell nuclear architecture across cell types in the mouse brain.
Takei Y, Zheng S, et al., Science (New York, N.Y.) 2021

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSTWADCJS
Biosample Biosource 
cerebral cortex - 4DNSR9Q964EF
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Confocal Laser Scanning
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch01
1-80
1267 loci (mouse) targeted by oligo probe library (with Alexa Fluor 647-labeled imaging oligos)
ch02
1-80
1193 loci (mouse) targeted by oligo probe library (with Cy3B-labeled imaging oligos)
ch03
1-80
1200 loci from 20 regions at 25-kb resolution (mouse) targeted by oligo probe library (with Alexa Fluor 488-labeled imaging oligos)
ch01
-
39 RNAs (mouse) targeted by oligo probe library (with Alexa Fluor 647-labeled imaging oligos)
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