ImagingPath
1267 loci (mouse) targeted by oligo probe library (with Alexa Fluor 647-labeled imaging oligos)

released

DNA seqFISH+ on 1267 loci in the 633-nm channel. Together with the 561-nm channel, a total of 2460 loci approximately 1-Mb apart are imaged. In each round of barcoding, 16 rounds of serial hybridizations are performed. Each locus is imaged once per barcoding round. In 5 barcoding rounds (80 rounds of hybridizations in total) each locus is imaged 5 times and the identity of all loci can be decoded.

   May 12th, 2022 at 9:45am

Details


experiment_type 
aliases 
  1. long-cai-lab:path_DNAseqFISHp_647
file_reference 
imaging_rounds 
1-80
labeled_probe 
imaging oligos
labels 
  1. Alexa Fluor 647
other_probes 
  1. oligo probe library
public_release 
June 27th, 2022
type_labeled_probe 
DNA primer