ExperimentMic multiplexed FISH on ES-E14TG2a.4

multiplexed FISH4DNEXM45AILX
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5 FOVs

   May 12th, 2022 at 9:46am
Source Publication
Integrated spatial genomics reveals global architecture of single nuclei.
Takei Y, Yun J, et al., Nature 2021

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSWJHV4QX
Biosample Biosource 
ES-E14TG2a.4 - 4DNSRSEQTR2T
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Confocal Laser Scanning
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch01
1-80
1267 loci (mouse) targeted by oligo probe library (with Alexa Fluor 647-labeled imaging oligos)
ch02
1-80
1193 loci (mouse) targeted by oligo probe library (with Cy3B-labeled imaging oligos)
ch03
1-80
1200 loci from 20 regions at 25-kb resolution (mouse) targeted by oligo probe library (with Alexa Fluor 488-labeled imaging oligos)
ch01
-
35 RNAs (mouse) targeted by oligo probe library (with Alexa Fluor 647-labeled imaging oligos)
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