ExperimentSetReplicate

ExperimentSet4DNESABVQJTS
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Chromatin tracing of a 2.5-Mb region on chromosome 21 (Chr21:34.6Mb-37.1Mb) in HCT116 cells with RAD21-AID, treated with auxin and after cell division (EdU+ and Geminin-)

   September 2nd, 2022 at 10:49am
Source Publication
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.
Bintu B, Mateo LJ, et al., Science (New York, N.Y.) 2018

Microscope configuration - Bintu et al 2018  

The experiments associated with this study are derived from either c8e8a284-c6d0-4b97-9670-822ae7a8296f or a2b6ca4e-7de9-484f-b8c3-c868b68f929e microscopes

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
HCT116 with Crispr generated mClover and AID-tagged RAD21
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
Chemical
Assay Details 
Target: 83 consecutive 30-kb loci on chr21, Geminin, EdU labeled Chromosomes
Channel
Imaging Rounds
Path
 
ch03
1-44
83 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch04
1-44
83 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch03
0
Geminin targeted by anti-geminin (with anti-Rabbit, Cy5)
ch01
0
EdU labeled Chromosomes targeted by Alexa Fluor 488
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXSHGF92P
Bio Rep 1, Tec Rep 1
multiplexed FISH on HCT116 with Crispr generated mClover and AID-tagged RAD21
File
4DNFIZNK1QQA
FOF-CT - DNA-spot/trace core (csv)
648.7 kB