ExperimentMic multiplexed FISH on HCT116 with Crispr generated mClover and AID-tagged RAD21

multiplexed FISH4DNEXSHGF92P
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   September 2nd, 2022 at 10:49am
Source Publication
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.
Bintu B, Mateo LJ, et al., Science (New York, N.Y.) 2018

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSPQL32HI
Biosample Biosource 
HCT116 with Crispr generated mClover and AID-tagged RAD21 - 4DNSRG1Q4WCR
Biosample Modifications 
None
Biosample Treatments 
IAA treatment (500.0 μM, 6.0h)
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
HARVARD-Zhuang_lab_CT-STORM6_Nikon_Eclipse_Ti-U_Scope1
Channel
Imaging Rounds
Path
 
ch03
1-44
83 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch04
1-44
83 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch03
0
Geminin targeted by anti-geminin (with anti-Rabbit, Cy5)
ch01
0
EdU labeled Chromosomes targeted by Alexa Fluor 488
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