ImagingPath
83 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)

released

Sequential DNA FISH of 44 loci in the 647-nm channel. Together with the 750-nm channel, a total of 83 consecutive 30-kb loci in a 2.5-Mb region on chromosome 21 (Chr21:34.6Mb-37.1Mb) are imaged by sequential hybridization of readout oligos complementary to primary oligo probes and labelled with fluorophore-conjugated imaging oligos.

   September 2nd, 2022 at 10:48am

Details


experiment_type 
aliases 
  1. alistair-boettiger-lab:path_2.5-Mb_AF647
contributing_labs 
imaging_rounds 
1-44
labeled_probe 
imaging oligos
labels 
  1. Alexa Fluor 647
other_probes 
  1. oligo probe library
  2. readout oligo library
public_release 
October 20th, 2022
type_labeled_probe 
DNA primer