ExperimentMic multiplexed FISH on IMR-90

multiplexed FISH4DNEXXNRAOTH
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Chromatin tracing on Primed human induced pluripotent stem cells IMR90 in mTeSR1 culture condition

   August 22nd, 2023 at 7:26pm
Source Publication
Female naive human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations.
Patterson B, Yang B, et al., Science advances 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSEQ8BDGJ
Biosample Biosource 
IMR-90 - 4DNSRXXGU3CH
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
1-20
Alexa Fluor 488-labeled fiducial beads
ch02
1-20
20 TADs chrX (1-20) targeted by Alexa Fluor 647-labeled imaging oligos
ch03
1-20
20 TADs chrX (21-40) targeted by ATTO 565-labeled imaging oligos

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