ExperimentType Bru-seq

   June 3rd, 2019 at 4:11pm
Reference Publication
Paulsen MT et al. (2014) PMID:23973811
Paulsen MT, Veloso A, et al., Methods (San Diego, Calif.) 2014


Experiment Category 
Assay Classification 
RNA Quantification
Experimental Purpose 
Raw Files Available 
Reads (fastq) provided by lab

Assay Description

Bru-Seq is a technique to detect spans of nascent transcription in the genome. Nascent RNA is labeled by a pulse of Bromouridine (BrU) and specifically captured using anti-BrdU antibodies. cDNA libraries are produced from the isolated Bru-RNA and sequenced. Sequences are aligned and analyzed to assess timing and level of transcription.