ExperimentSetReplicate

ExperimentSet4DNESYTUBW2E
released

Hi-C on h1ESC cells with HindIII

   July 9th, 2020 at 10:45pm
Source Publication
Systematic evaluation of chromosome conformation capture assays.
Akgol Oksuz B, Yang L, et al., Nature methods 2021

WARNING: non-optimal cross-linking conditions  


This dataset may be obtained with non-optimal cross-linking conditions (as demonstrated in the paper) and is reported here for documentation purposes.

Users interested in using Hi-C or Micro-C data from this project for alternative analyses are advised to use only datasets generated with the optimal cross-linking conditions of 1% Formaldehyde + 3mM DSG. The study authors further advise users to carefully select digestion enzymes and library size to fit their needs. A list of preferred datasets for re-use is provided.

Note: Superseding Item - 4DNESYTUBW2E  


This experiment set is replacing 4DNES78Y8Y5K. The experiment type was initially submitted as "Dilution Hi-C" but it was subsequently determined that the correct experiment type is "in situ Hi-C". In order to correct this mistake, the old Experiment Set and associated Experiment have been replaced with new ones of the correct type. This correction does not affect any file or file processing pipeline associated with this data set. Both raw and processed files linked to this experiment or the associated items remain unchanged.

Assay Description  



Experiment Set Properties  


Set Type 
Replicate
Organism 
Sample Type 
stem cells
Sample 
Experiment Type(s) 
Modification Type 
None
Treatment Type 
None
Assay Details 
Enzyme: HindIII

8 Processed Files

Quality Metrics

Experiment
For File
Filtered Reads
Cis reads (>20kb)
Short cis reads
Trans Reads
Report
Details
From Multiple Experiments
4DNESYTUBW2E
Multiple Experiments
File
Contact List-combined (pairs)
4DNFI733IUMB
179.59m
46.7%
22.919%
30.38%
Experiment
4DNEXP6V97PN
Bio Rep 1, Tec Rep 1
in situ Hi-C on H1-hESC with HindIII
File
Contact List-replicate (pairs)
4DNFIHGRXQWZ
179.59m
46.7%
22.919%
30.38%
Experiment
For File
Failed Balancing
Details
From Multiple Experiments
4DNESYTUBW2E
Multiple Experiments
File
Contact Matrix (mcool)
4DNFISWCOKN2
None
Experiment
For File
Total Reads
Unmapped Reads
Multimapped Reads
Duplicate Reads
Walks
Minor Contigs
Details
Experiment
4DNEXP6V97PN
Bio Rep 1, Tec Rep 1
in situ Hi-C on H1-hESC with HindIII
File
Alignments (bam)
4DNFIYPLRRSZ
383.12m
13.153%
14.412%
25.558%
0.002%
0.075%