ExperimentSetReplicate

ExperimentSet4DNESW3L596Q
released

WARNING: one replicate with input mapping bias - Replicates of SON Ab2 TSA-seq version 2 Reaction Condition 2 (PBS 50% Sucrose) Enhancement condition E (1:300 tyramide-biotin, 30 minute reaction) on K562 cells

   July 1st, 2019 at 2:42am

TSA-seq Replicate Set with Biased Input Mapping  


WARNING: One experiment in this set of replicates displays biased input mapping.

"Biased input" refers to input data sets that show unexpectedly large variations in read count over large chromosome domains that correlate with GC content as well as LAD/inter-LADs and early versus late DNA replication domains. They appear to be related to PCR bias during library construction. Input profiles typically are very similar from the same cell line between different experiments. TSA-Seq scores computed using input data from other experiments from the same cell line typically produce TSA-Seq scores significantly closer to TSA-Seq scores from replicate data sets which do not have biased input.


Experiment Set Properties  


Set Type 
Replicate
Organism 
Sample Type 
immortalized cells
Experiment Type(s) 
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: SON protein

2 Processed Files