ExperimentSetReplicate

ExperimentSet4DNESVDDCXBV
released
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Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on a volume the size of the nucleus. Test of imaging immunofluorescence and FISH probes simultaneously.

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Assay Description  

Note on Localization h5 Processed Files  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
primary cells
Sample 
IMR-90 (Tier 1)
Experiment Type(s) 
DNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Human chr13 LAD (pool 2), LaminA/C protein
Sample Image 
Sample frame of rendered image - chr13 LAD (red) and Lamin A/C (green)
View File Item - 4DNFIQFW2AZO
Channel
Path
 
ch00
Human chr13 LAD (pool 2) targeted by Alexa Fluor 647
ch01
LaminA/C protein targeted by anti-Lamin A/C, biotin-labeled anti-Mouse (with CF568-conjugated streptavidin (Biotium, 29035))

1 Processed File

Experiment
File
File Type
File Size
Notes
Experiment
4DNEX2VUG2MU
Bio Rep 1, Tec Rep 1
DNA FISH on IMR-90 (Tier 1)
File
4DNFI6OD1VR4
localizations (hdf5)
1.47 MB