ExperimentMic DNA FISH on IMR-90 (Tier 1)

DNA FISH4DNEX2VUG2MU
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Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on a volume the size of the nucleus. Test of imaging immunofluorescence and FISH probes simultaneously.

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Properties  

Experiment Type 
DNA FISH
Follows SOP 
No
Biosample 
4DNBSZQYOXTJ
Biosample Biosource 
IMR-90 (Tier 1) - 4DNSRI51KRED
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
STORM
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Human chr13 LAD (pool 2) targeted by Alexa Fluor 647
642nm
ch01
LaminA/C protein targeted by anti-Lamin A/C, biotin-labeled anti-Mouse (with CF568-conjugated streptavidin (Biotium, 29035))
560nm

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