ExperimentSetReplicate

ExperimentSet4DNESTTO3H2N
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Multiplexed FISH (ORCA) in wild-type double positive (DP) T-cells derived from C57BL-6 mice to target Ets1-Fli1 locus

   June 3rd, 2024 at 8:20pm
Source Publication
Single-Allele Chromatin Tracing Reveals Genomic Clustering of Paralogous Transcription Factors as a Mechanism for Developmental Robustness in T Cells.
Jay A, Zhou Y, et al., bioRxiv : the preprint server for biology 2025

Data Usage Guidelines  

Data Use Guidelines: This is a data set generated by the 4DN Network and made freely available to the scientific community. If you are intending to use these data for a publication, we ask that you please contact the data generating lab to discuss possible coordinated publication. In your manuscript, please cite the 4DN White Paper (doi:10.1038/nature23884) and the 4DN Data Portal paper (doi:10.1038/s41467-022-29697-4), and please acknowledge the 4DN lab which generated the data. Please direct any questions to the Data Coordination and Integration Center.

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
primary cells
Sample 
double-positive, alpha-beta thymocyte
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Ets1 mouse gene
Channel
Imaging Rounds
Path
 
ch00
1-60
900-kb region around Ets1-Fli1 locus targeted by Cy3-labeled fiducial probes (reference fiducial channel)
ch01
1-60
900-kb region around Ets1-Fli1 locus targeted by Alexa Fluor 647 labeled readout probes (readout channel)

2 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXSTYC535
Bio Rep 1, Tec Rep 1
multiplexed FISH on double-positive, alpha-beta thymocyte
File
4DNFI5OF5LWK
FOF-CT - DNA-spot/trace core (csv)
5.73 MB
File
4DNFIMPCT3OK
FOF-CT - spot quality (csv)
7.56 MB