ExperimentSetReplicate

ExperimentSet4DNESKWEG12C
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Chromatin tracing at chromosome X H7 day 2 in 5iLA culture condition combined with XIST RNA FISH (XIST- chromosomes)

   August 22nd, 2023 at 7:15pm
Source Publication
Female naive human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations.
Patterson B, Yang B, et al., Science advances 2023

Microscope configuration  

The experiments associated with this study are derived from either d8f51a9c-1e1a-4a6c-9432-ddf7de64e836 or f0172a96-2bf5-4e6a-938d-f205c6e728c9 microscopes

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
stem cells
Sample 
H7-hESC
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 20 TADs chrX (1-20), 20 TADs chrX (21-40), XIST transcript
Channel
Imaging Rounds
Path
 
ch00
1-20
Alexa Fluor 488-labeled fiducial beads
ch02
1-20
20 TADs chrX (1-20) targeted by Alexa Fluor 647-labeled imaging oligos
ch03
1-20
20 TADs chrX (21-40) targeted by ATTO 565-labeled imaging oligos
ch01
-
XIST transcript targeted by Cy3-labeled imaging oligos

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXPM5YBYG
Bio Rep 1, Tec Rep 1
multiplexed FISH on H7-hESC
File
4DNFI9BJFNFL
FOF-CT - DNA-spot/trace core (csv)
407.15 kB