ExperimentSetReplicate

ExperimentSet4DNES1IC1QNZ
released
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FISH/IF of Chromosome 3-Control and Chromosome 15-NOR and Nucleolus

   May 5th, 2018 at 4:04am
Source Publication
Higher-Order Inter-chromosomal Hubs Shape 3D Genome Organization in the Nucleus.
Quinodoz SA, Ollikainen N, et al., Cell 2018

Assay Description  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
F1 2-1 mESC line
Experiment Type(s) 
DNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Chromosomes, GRCm38:3:153337160-154337181 mouse region, GRCm38:15:4050417-6048847 mouse region, Ncl mouse protein
Sample Image 
DNA FISH with DAPI and Nucleolin costaining
View File Item - 4DNFIO7UUCRI
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
GRCm38:3:153337160-154337181 mouse region targeted by 495/517nm fluorophore-labeled oligo probe set
ch02
GRCm38:15:4050417-6048847 mouse region targeted by 547/565nm fluorophore-labeled oligo probe set
ch03
Ncl mouse protein targeted by rabbit anti-nucleolin antibody (with Alexa Fluor 647-labeled anti-rabbit secondary antibody)

3 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXQA2H161
Bio Rep 1, Tec Rep 1
DNA FISH on F1 2-1 mESC line
File
4DNFI41IIITG
locus distances, submitter format (csv)
2.05 kB
File
4DNFIZQ7EBAN
locus distances, submitter format (csv)
2.09 kB
File
4DNFIY2LPCJB
locus distances, submitter format (csv)
1.56 kB