Publication
Krietenstein N et al. (2020) Ultrastructural Details of Mammalian Chromosome Architecture.

current
   March 30th, 2020 at 3:23pm

Overview


Abstract

Over the past decade, 3C-related methods have provided remarkable insights into chromosome folding in vivo. To overcome the limited resolution of prior studies, we extend a recently developed Hi-C variant, Micro-C, to map chromosome architecture at nucleosome resolution in human ESCs and fibroblasts. Micro-C robustly captures known features of chromosome folding including compartment organization, topologically associating domains, and interactions between CTCF binding sites. In addition, Micro-C provides a detailed map of nucleosome positions and localizes contact domain boundaries with nucleosomal precision. Compared to Hi-C, Micro-C exhibits an order of magnitude greater dynamic range, allowing the identification of approximately 20,000 additional loops in each cell type. Many newly identified peaks are localized along extrusion stripes and form transitive grids, consistent with their anchors being pause sites impeding cohesin-dependent loop extrusion. Our analyses comprise the highest-resolution maps of chromosome folding in human cells to date, providing a valuable resource for studies of chromosome organization.

Authors

Krietenstein N  •  Abraham S  •  Venev SV  •  Abdennur N  •  Gibcus J  •  Hsieh TS  •  Parsi KM  •  Yang L  •  Maehr R  •  Mirny LA  •  Dekker J  •  Rando OJ

Link

https://www.ncbi.nlm.nih.gov/pubmed/32213324


Journal

Molecular cell

PMID:32213324

Published

March 14th, 2020