Structural elements facilitate extreme long-range gene regulation at a human disease locus

   October 27th, 2022 at 8:00am

Note: Replaced Biorxiv  

This biorxiv set was replaced by PMID:36996812.



Enhancer clusters overlapping disease-associated mutations in Pierre Robin sequence (PRS) patients regulate SOX9 expression at genomic distances over 1.25 megabases. We applied optical reconstruction of chromatin architecture (ORCA) imaging to trace 3D locus topology during PRS-enhancer activation. While we observed pronounced changes in locus topology between cell-types, analysis of single chromatin fiber traces revealed that these ensemble-average differences arise not from the presence of cell-type unique conformations, but through changes in frequency of commonly sampled topologies. We further identified two CTCF-bound elements, internal to the SOX9 topologically associating domain, which are positioned near its 3D geometric center and bridge enhancer-promoter contacts in a series of chromatin loops. Ablation of these elements results in diminished SOX9 expression and altered domain-wide contacts. Polymer models with uniform loading across the domain and frequent cohesin collisions recapitulate this multiloop, centrally clustered geometry, suggesting a mechanism for gene regulation over ultralong ranges. Four short bullet points that convey the key message of the paperSOX9 domain topology dynamically changes during a developmental transition Structural elements promote TAD-wide interactions, stripe formation and transcription Structural elements are CTCF-dependent and situated centrally in the 3D TAD structure Polymer simulations of multi-loop model best recapitulate topological features


Chen L-F  •  Long HK  •  Park M  •  Swigut T  •  Boettiger AN  •  Wysocka J






October 21st, 2022