Mice cloned from olfactory sensory neurons.

Abstract

Cloning by nuclear transplantation has been successfully carried out in various mammals, including mice. Until now mice have not been cloned from post-mitotic cells such as neurons. Here, we have generated fertile mouse clones derived by transferring the nuclei of post-mitotic, olfactory sensory neurons into oocytes. These results indicate that the genome of a post-mitotic, terminally differentiated neuron can re-enter the cell cycle and be reprogrammed to a state of totipotency after nuclear transfer. Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice.

Authors

Eggan K  •  Baldwin K  •  Tackett M  •  Osborne J  •  Gogos J  •  Chess A  •  Axel R  •  Jaenisch R

Link

https://www.ncbi.nlm.nih.gov/pubmed/14990966