Dynamic reorganization of nuclear architecture during human cardiogenesis

Abstract

While chromosomal architecture varies among cell types, little is known about how this organization is established or its role in development. We integrated Hi-C, RNA-seq and ATAC-seq during cardiac differentiation from human pluripotent stem cells to generate a comprehensive profile of chromosomal architecture. We identified active and repressive domains that are dynamic during cardiogenesis and recapitulate in vivo cardiomyocytes. During differentiation, heterochromatic regions condense in cis . In contrast, many cardiac-specific genes, such as TTN (titin), decompact and transition to an active compartment coincident with upregulation. Moreover, we identify a network of genes, including TTN , that share the heart-specific splicing factor, RBM20, and become associated in trans during differentiation, suggesting the existence of a 3D nuclear splicing factory. Our results demonstrate both the dynamic nature in nuclear architecture and provide insights into how developmental genes are coordinately regulated. One Sentence Summary The three-dimensional structure of the human genome is dynamically regulated both globally and locally during cardiogenesis.

Authors

Paul A. Fields  •  Vijay Ramani  •  Giancarlo Bonora  •  Gurkan Yardimci  •  Alessandro Bertero  •  Hans Reinecke  •  Lil Pabon  •  William S. Noble  •  Jay Shendure  •  Charles E. Murry

Link

https://www.biorxiv.org/content/10.1101/222877v1