Publication

ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide.

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   January 24th, 2018 at 3:09pm

Overview

Abstract

This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as to map regions of transcription-factor binding and nucleosome position. The method is a fast and sensitive alternative to DNase-seq for assaying chromatin accessibility genome-wide, or to MNase-seq for assaying nucleosome positions in accessible regions of the genome.

Authors

Buenrostro JD  •  Wu B  •  Chang HY  •  Greenleaf WJ

Link

https://www.ncbi.nlm.nih.gov/pubmed/25559105

Journal

Current protocols in molecular biology

PMID:25559105

Published

January 5th, 2015

Category

methods paper