Protocol
H1toDEdifferentiation.png

released

Authentication of human Embryonic Stem Cells (H1) and Definitive Endoderm (DE) Markers for 02/13/18 CUT & RUN Experiment. (A) Immuno-staining confirms that following 5 days of DE differentiation Oct4 is downregulated in DE cells relative to H1 cells, (B & C) and FoxA2 and Sox17 are upregulated at the protein level. Phalloidin (Phall) marks the cell cortex, and DAPI marks the nucleus in the overlayed images. (D) qPCR analysis confirms that following 5 days of DE differentiation Oct4 is downregulate and FoxA2 and Sox17 are upregulated in DE cells relative to H1 cells at the mRNA level.

   April 25th, 2018 at 5:01pm

Overview


Protocol Type 
Authentication document
Protocol Classification 
Differentiation Authentication
Attachment 
View H1toDEdifferentiation.png
MD5  : 68873501364a522064a55b63e05f2fa0
File Type  : image/png