OntologyTerm Hi-C

released
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   May 11th, 2017 at 3:50pm

Details

definition 
Hi-C is a method for analysing 3-dimensional organisation and interaction of chromatin, belonging to the class of techniques collectively called "Chromosome Conformation Capture", in which chromatin is crosslinked, then digested, and re-ligated in such a way that only DNA fragments that are covalently linked together form ligation products. Together with 5C, Hi-C does not limit to the study to a specific locus in the genome, and can be used to study chromatin interaction across the genome. To achieve high-throughput, a biotin-labeled nucleotide is incorporated at the ligation junction, enabling selective purification of chimeric DNA ligation junctions followed by deep sequencing. Mapping the sequenced reads back to genome will reveal the origin of the fragments involved in the ligation events. (EFO)
namespace 
http://www.ebi.ac.uk/efo
parents 
  1. assay by sequencer
  2. ligation-free chromosome conformation capture assay
preferred_name 
Hi-C
references 
  1. None
source_ontologies 
  1. Experimental Factor Ontology
term_id 
EFO:0007693
term_name 
Hi-C
term_url 
http://www.ebi.ac.uk/efo/EFO_0007693