OntologyTermH9

released
   May 11th, 2017 at 3:56pm

Details


definition 
This human ES cell line expresses cell surface markers specific to undifferentiated nonhuman primate ES and human EC cells: stage-specific embryonic antigen (SSEA)–3, SSEA-4, TRA-l-60, TRA-1- 81, and alkaline phosphatase. It has a normal 46, XX karyotype. Teratomas are formed upon cell injection into SCID mice. These teratomas include cells from all three germ layers. Isolation & Growth Conditions This cell line can be cultured using feeder cells or in a feeder-free protocol. When using feeder cells, human ESC culture medium should be compreised of Advanced DMEM/F12 supplemented with knockout serum replacement (20%), non-essential amino acids (1x), L-glutamine (1x), penicillin/streptomycin (1x), β-mercaptoethanol (1x) and FGF-2 (4 ng/ml). Maintain the human ESCs on Matrigel using hESC medium conditioned by mouse embryonic fibroblasts. (EFO)
namespace 
http://www.ebi.ac.uk/efo
preferred_name 
H9
references 
  1. None
slim_terms 
source_ontologies 
term_id 
EFO:0003045
term_name 
H9
term_url 
http://www.ebi.ac.uk/efo/EFO_0003045