ImagingPath
28 consecutive 30-kb loci on chrX (closed chromatin) targeted by oligo probe library (with ATTO 565-labeled imaging oligos)

released

Sequential DNA FISH of 14 loci in the ATTO 565 channel. Together with the Alexa Fluor 647 channel, a total of 28 consecutive loci at 30-kb resolution in a closed chromatin region of chrX (chrX:8360000-9200000, GRCh38) is imaged by sequential hybridization of fluorophore-conjugated imaging oligos complementary to primary oligo probes.

   August 3rd, 2022 at 10:58am

Details


experiment_type 
aliases 
  1. siyuan-wang-lab:path_closed_library_readout_atto565
imaging_rounds 
1-14
labeled_probe 
imaging oligos
labels 
  1. ATTO 565
other_probes 
  1. oligo probe library
public_release 
August 8th, 2022
type_labeled_probe 
DNA primer