ImagingPath
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)

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Sequential DNA FISH of 52 steps of 5-kb at the HoxA locus by sequential hybridization of readout oligos complementary to primary oligo probes and labelled with AF750-conjugated imaging oligos

   January 24th, 2023 at 12:48pm

Details

experiment_type 
DNA FISH
aliases 
  1. alistair-boettiger-lab:path_750_5kb_HoxA_chromatin_tracing
file_reference 
  1. 4DNFII68SPRW.bed.gz
  2. 4DNFIZQ2XYI1.fasta
imaging_rounds 
1-52
labeled_probe 
imaging oligos
labels 
  1. Alexa Fluor 750
other_probes 
  1. oligo probe library
  2. readout oligo library
public_release 
April 13th, 2023
target 
  1. 52 consecutive 5-kb regions at HoxA locus (mouse)
type_labeled_probe 
DNA primer