ImagingPath
consecutive 10-kb regions at Pidx1 locus (mouse) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)

released
View JSON

Sequential DNA FISH of 75 consecutive 10-kb steps at the Pitx1 locus by sequential hybridization of readout oligos complementary to primary oligo probes and labelled with fluorophore-conjugated imaging oligos

   September 29th, 2022 at 7:33am

Details

experiment_type 
DNA FISH
aliases 
  1. alistair-boettiger-lab:path_647_probe_library_1-75
contributing_labs 
  1. David Kingsley, STANFORD
file_reference 
  1. 4DNFIP283THX.bed.gz
  2. 4DNFIQBGXYA8.fasta
imaging_rounds 
1-75
labeled_probe 
imaging oligos
labels 
  1. Cy5
other_probes 
  1. oligo probe library
  2. readout oligo library
public_release 
February 8th, 2023
target 
  1. consecutive 10-kb regions at Pidx1 locus (mouse)
type_labeled_probe 
DNA primer