ImagingPath
80 spaced regions at SOX9 locus targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)

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Sequential DNA FISH of 52 steps (steps #7-58 of the oligopaint library targeting the 4-Mb SOX9 locus) of 5-kb, spaced by 50-kb (thus covering 2.6 Mb), at the SOX9 locus by sequential hybridization of readout oligos complementary to primary oligo probes and labelled with fluorophore-conjugated imaging oligos

   January 18th, 2023 at 9:24pm

Details

experiment_type 
DNA FISH
aliases 
  1. alistair-boettiger-lab:path_Cy5_sox9_7-58
contributing_labs 
  1. Joanna Wysocka, STANFORD
file_reference 
  1. 4DNFISMIUJ54.bed.gz
  2. 4DNFIE44K3LM.fasta
imaging_rounds 
7-58
labeled_probe 
imaging oligos
labels 
  1. Cy5
other_probes 
  1. oligo probe library
  2. readout oligo library
public_release 
February 13th, 2023
target 
  1. 80 spaced regions at SOX9 locus
type_labeled_probe 
DNA primer