Cells were arrested in G2 to synchronize, then released from arrest and fixed at several time points. Later timepoints were treated with nocodazole to prevent metaphase to anaphase transition. From left to right, top then bottom: G2 (0m); early prophase (5m); late prophase (10m); early prometaphase (15m); prometaphase (30m, blocked with nocodazole); prometaphase (60m, blocked with nocodazole). Note the large-scale changes in contact frequencies as mitosis begins.
October 15th, 2019 at 3:48pm