ExperimentMic SPT on Drosophila embryo

SPT4DNEXJFL4TTZ
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Single particle tracking experiment on a Drosophila embryo carrying the construct parS-homie-evePr-PP7, 142kb upstream to the eveMS2 allele. The embryo for imaging was obtained by crossing male flies carrying this construct with female flies of genotype MCP::3xtagBFP/PCP::3xmKate2; parB::eGFP/+.

   January 13th, 2021 at 11:42pm
Source Publication
Dynamic interplay between enhancer-promoter topology and gene activity.
Chen H, Levo M, et al., Nature genetics 2018

Properties  

Experiment Type 
SPT
Follows SOP 
No
Biosample 
4DNBSL9JDUI3
Biosample Biosource 
Drosophila embryo - 4DNSR2K5JQ6X
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Confocal Laser Scanning
Microscope Quality Control 
None
Microscope Configuration 
None
Sample Image 
Sample image snapshot from live cell imaging of a Drosophila embryo with parS-homie-evePr-PP7 eveMS2
View File Item - 4DNFIFVW96UY
Channel
Path
 
ch00
MS2-tagged endogenous eve transcript and enhancer (fruit fly) targeted by mTagBFP2-labeled MCP
405nm
ch01
PP7-tagged transcript from homie-evePromoter cassette (engineered reagent) targeted by mKate2-labeled PCP
591nm
ch02
ParS-tagged homie-evePromoter cassette (engineered reagent) targeted by eGFP-labeled ParB
488nm

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