ExperimentMic multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE

multiplexed FISH4DNEXZRASVLC
released

Imaging the 7.5kb insert to visualize chromatin structural changes in the presence of the synthetic insulator at the Sox2 locus known to reduce transcription of the Sox2 gene. We also colocalized the chromatin data with nascent RNA images to understand bursting behavior as a function of the allele classification.

   October 14th, 2021 at 3:32pm

Properties  


Experiment Type 
Follows SOP 
No
Biosample 
Biosample Modifications 
RMCE replacement
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None

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