ExperimentMic multiplexed FISH on limb bud

multiplexed FISH4DNEXYO4UN1U
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Chromatin tracing of Pidx1 locus (barcodes 1-68) in limb buds (right) from a mouse embryo at stage E11.5

   September 29th, 2022 at 7:33am
Source Publication
Boundary stacking interactions enable cross-TAD enhancer-promoter communication during limb development.
Hung TC, Kingsley DM, et al., Nature genetics 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSZTCM5EN
Biosample Biosource 
limb bud - 4DNSRU7AMKBU
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
Stanford-Boettiger_lab_ORCA_Nikon_Eclipse_Ti-U_Scope1
Channel
Imaging Rounds
Path
 
ch00
1-68
consecutive 10-kb regions at Pidx1 locus (mouse) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
1-68
consecutive 10-kb regions at Pidx1 locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)

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