ExperimentMic multiplexed FISH on IMR-90

multiplexed FISH4DNEXXQVNRK6
released
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   September 2nd, 2022 at 10:48am
Source Publication
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.
Bintu B, Mateo LJ, et al., Science (New York, N.Y.) 2018

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSICDLGNW
Biosample Biosource 
IMR-90 - 4DNSRXXGU3CH
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
HARVARD-Zhuang_lab_CT-STORM6_Nikon_Eclipse_Ti-U_Scope1
Channel
Imaging Rounds
Path
 
ch03
1-41
65 consecutive 30-kb loci on chr21 targeted by oligo probe library (with Alexa Fluor 647-labeled readout oligos)
ch03
42-65
65 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch01
0
Geminin targeted by anti-geminin (with anti-Rabbit, Alexa Fluor 488)
ch00
-
Chromosomes targeted by DAPI
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