ExperimentMic multiplexed FISH on type II pneumocyte

multiplexed FISH4DNEXXQBDXWI
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Multiplexed FISH in KP primary adenocarcinoma cancer cells where Rnf2 is degraded using dTAG-13 via FKBP12F36V degron tagged to the C-terminus of Rnf2 from mouse, Biological Relicate 1, Technical Replicate 1

   May 27th, 2025 at 5:42pm
Source Publication
Tracing the evolution of single-cell cancer 3D genomes: an atlas for cancer gene discovery.
Liu M, Jin S, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBS8QUHJ8Y
Biosample Biosource 
type II pneumocyte - 4DNSRA37IMS3
Biosample Modifications 
None
Biosample Treatments 
dTAG-13 treatment (30m)
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Light yellow fiducial beads for drift correction
ch01
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by Alexa 647-labelled imaging oligos
ch02
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by ATTO 565-labelled imaging oligos
ch03
0
Chromosomes targeted by DAPI

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