ExperimentMic multiplexed FISH on Adenoma from mouse SA3961 (fine-scale)

multiplexed FISH4DNEXTZPG7H7
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Multiplexed FISH fine-scale in dtTomato+ GFP+ expressing (yellow) heterozygous mutant (Trp53 +/-) adenoma cells from K-MADM-Trp53 mouse, Biological Relicate 1, Technical Replicate 1

   May 27th, 2025 at 5:41pm
Source Publication
Tracing the evolution of single-cell cancer 3D genomes: an atlas for cancer gene discovery.
Liu M, Jin S, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSBDUAH34
Biosample Biosource 
Adenoma from mouse SA3961 (fine-scale) - 4DNSRAFFC2LM
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Yellow-green fiducial beads for drift correction
ch01
0
Chromosomes targeted by DAPI
ch02
1-50
40 consecutive 5-kb to 20-kb loc in each of the 10 genes targeted by Alexa fluor 647 imaging oligos
ch03
1-50
40 consecutive 5-kb to 20-kb loci in each of the 10 genes targeted by Atto 560 imaging oligos
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