ExperimentMic multiplexed FISH on double-positive, alpha-beta thymocyte

multiplexed FISH4DNEXSTYC535
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Multiplexed FISH (ORCA) in wild-type double positive (DP) T-cells derived from C57BL-6 mice to target Ets1-Fli1 locus, Biological replicate 1, technical replicate 1

   June 3rd, 2024 at 8:20pm
Source Publication
Single-Allele Chromatin Tracing Reveals Genomic Clustering of Paralogous Transcription Factors as a Mechanism for Developmental Robustness in T Cells.
Jay A, Zhou Y, et al., bioRxiv : the preprint server for biology 2025

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSDB4CAC8
Biosample Biosource 
double-positive, alpha-beta thymocyte - 4DNSRNWQR9Y3
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
no view permissions
Channel
Imaging Rounds
Path
 
ch00
1-60
900-kb region around Ets1-Fli1 locus targeted by Cy3-labeled fiducial probes (reference fiducial channel)
ch01
1-60
900-kb region around Ets1-Fli1 locus targeted by Alexa Fluor 647 labeled readout probes (readout channel)

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