ExperimentMic RNA FISH on Fruit fly (Oregon-R) embryo - NC13

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RNA FISH4DNEXRJCTDA8
released
View JSON
   November 5th, 2019 at 8:54am
Source Publication
Diverse Spatial Expression Patterns Emerge from Unified Kinetics of Transcriptional Bursting.
Zoller B, Little SC, et al., Cell 2018

Properties  

Experiment Type 
RNA FISH
Follows SOP 
No
Biosample 
4DNBSF9PCSD8
Biosample Biosource 
Fruit fly (Oregon-R) embryo - NC13 - 4DNSRFY2Y29L
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Confocal Laser Scanning
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
5' region of knuppel mRNA (fruit fly) targeted by ATTO 565-labeled custom DNA oligos
ch02
3' region of knuppel mRNA (fruit fly) targeted by ATTO 633-labeled custom DNA oligos

1 Experiment SetOpen In Search View

Title
Set Type
Experiment Type
Biosample
Dataset
Condition
Assay Details
Lab
4DNES79RMWNZ
Replicate
RNA FISH
Fruit fly (Oregon-R) embryo - NC13
Dual color smFISH on fruit fly embryo (nuclear cycle 13)
Target: wt kruppel mRNA
Target
8' region of knuppel mRNA (fruit fly), Chromosomes
Thomas Gregor, PRINCETON

Single molecule mrnaFISH on endogenous Krüppel using dual color 5' and 3' block probe sets

Lab:
Thomas Gregor, PRINCETON
Publication:
Zoller B et al. (2018) PMID:30340044

11 Processed Files

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