ExperimentMic multiplexed FISH on primary motor cortex

multiplexed FISH4DNEXRBK6HWZ
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Genome-wide chromatin imaging by DNA-MERFISH of 2000 loci in mouse primary motor cortex of Mecp2 heterozygous knock-out mice, together with RNA-MERFISH of 240 gene transcripts, Biological replicate 3, Technical replicate 1

   November 15th, 2023 at 9:30pm
Source Publication
Cell-type-specific 3D-genome organization and transcription regulation in the brain.
Liu S, Zheng P, et al., bioRxiv : the preprint server for biology 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSQRAB6W7
Biosample Biosource 
primary motor cortex - 4DNSRSHTV6AX
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch02
13-97
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Cy3-labeled imaging oligos)
ch03
13-97
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch04
13-97
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch01
0
540/560nm fiducial beads
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