ExperimentMic multiplexed FISH on mouse embryonic stem cell

multiplexed FISH4DNEXPUOPS98
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Multiplexed FISH in mouse embryonic stem cell at NANOG locus, differentiated for 2 days, Biological Replicate 2 ,Technical Replicate 1 (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).

   February 4th, 2025 at 9:45pm
Source Publication
Single-cell chromatin state transitions during epigenetic memory formation.
Fujimori T, Rios-Martinez C, et al., bioRxiv : the preprint server for biology 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSLN29GS1
Biosample Biosource 
mouse embryonic stem cell - 4DNSRB24NZT3
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
-
DAPI staining
ch01
1-19
~150-kb region around Nanog promoter targeted by by Cy5-labeled imaging oligos
ch02
1-19
~150-kb region around Nanog promoter targeted by Cy3-labeled fiducial oligos.

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