ExperimentMic multiplexed FISH on IMR-90

multiplexed FISH4DNEXOCO3JFI
released
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   August 3rd, 2022 at 10:59am
Source Publication
TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations.
Cheng Y, Liu M, et al., Genome biology 2021

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSXEDQE7K
Biosample Biosource 
IMR-90 - 4DNSRXXGU3CH
Biosample Modifications 
None
Biosample Treatments 
Trichostatin A treatment (100.0 ng/mL, 24.0h) and Sodium butyrate treatment (20.0 nM, 24.0h)
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
Yale-Wang_lab_MINA_Nikon_Eclipse_Ti2-U_Jellyfish_Config2
Channel
Imaging Rounds
Path
 
ch03
-
28 consecutive 30-kb loci on chrX targeted by Cy5-labeled oligo probe library
ch02
-
XIST transcript targeted by ATTO 565-labeled oligo probes
ch03
1-14
28 consecutive 30-kb loci on chrX targeted by oligo probe library (with Alexa Fluor 647-labeled imaging oligos)
ch02
1-14
28 consecutive 30-kb loci on chrX targeted by oligo probe library (with ATTO 565-labeled imaging oligos)
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