ExperimentMic multiplexed FISH on visual cortex

multiplexed FISH4DNEXO6XHKYI
released
View JSON

Genome-wide chromatin imaging by DNA-MERFISH of 2000 loci in mouse visual cortex of Mecp2 heteozygous knock-out mice, together with RNA-MERFISH of 240 gene transcripts, Biological replicate 1, Technical replicate 1. Fluorophore cleavage and photobleaching (for DNA-MERFISH) used as extinguishing method for the experiment.

   July 22nd, 2024 at 9:03pm
Source Publication
Cell type-specific 3D-genome organization and transcription regulation in the brain.
Liu S, Wang CY, et al., Science advances 2025

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSSVSWCRA
Biosample Biosource 
visual cortex - 4DNSRTE7XU6W
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
Harvard-Zhuang_lab_CT-STORM_Nikon_Eclipse_Ti-U_Liu-2023_Scope1
Channel
Imaging Rounds
Path
 
ch02
12-87
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Cy3-labeled imaging oligos)
ch03
12-87
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch04
12-87
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch01
0
540/560nm fiducial beads
Show 4 More

Experiment SetOpen In Search View

Initializing...