ExperimentMic multiplexed FISH on type II pneumocyte

multiplexed FISH4DNEXNIH3M1J
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Multiplexed FISH in mutant Rnf2 rescue cell line generated by knockdown of Rnf2 via small hairpin RNA (shRnf2) followed by insertion of vector expressing catalytic Rnf2 mutant (I53S) lacking ubiquitin ligase activity in KP primary adenocarcinoma cancer cells from mouse, Biological Relicate 2, Technical Replicate 1

   May 27th, 2025 at 5:42pm
Source Publication
Tracing the evolution of single-cell cancer 3D genomes: an atlas for cancer gene discovery.
Liu M, Jin S, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSQZE3LFK
Biosample Biosource 
type II pneumocyte - 4DNSRA37IMS3
Biosample Modifications 
Stable Transfection disruption for Rnf2 mouse gene
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Light yellow fiducial beads for drift correction
ch01
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by Alexa 647-labelled imaging oligos
ch02
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by ATTO 565-labelled imaging oligos
ch03
0
Chromosomes targeted by DAPI

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