ExperimentMic RNA FISH on G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion

RNA FISH4DNEXM55PA4P
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RNA FISH of Hbaa1, Prdx2 and Klf1 intronic regions in G1E-ER4 cells differentiated for 24 hours treated for 60min with 250nM JQ1

   September 14th, 2018 at 4:52pm
Source Publication
Transcriptional Burst Initiation and Polymerase Pause Release Are Key Control Points of Transcriptional Regulation.
Bartman CR, Hamagami N, et al., Molecular cell 2018

Properties  

Experiment Type 
RNA FISH
Follows SOP 
No
Biosample 
4DNBSMJNDHD3
Biosample Biosource 
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion - 4DNSR41SLMH6
Biosample Modifications 
None
Biosample Treatments 
estradiol treatment (100 nM, 24h) and JQ1 treatment (250.0 nM, 60.0m)
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
GRCm38:11:32283672-32284486 mouse region targeted by Alexa Fluor 594-labeled custom oligos
ch02
GRCm38:8:84969622-84974811 mouse region targeted by Cy5-labeled custom oligos
ch03
GRCm38:8:84901928-84905295 mouse region targeted by Cy3-labeled custom oligos

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