ExperimentMic multiplexed FISH on Adenoma from mouse SA3961 and Adenoma from mouse SA3506 and Adenoma from mouse SA3441 and Adenoma from mouse SA3962

multiplexed FISH4DNEXKPXSCHD
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Multiplexed FISH in dtTomato-expressing (red) homozygous WT (Trp53 +/+) adenoma cells from K-MADM-Trp53 mouse, Biological Replicate 1, Technical Replicate 1

   May 27th, 2025 at 5:41pm
Source Publication
Tracing the evolution of single-cell cancer 3D genomes: an atlas for cancer gene discovery.
Liu M, Jin S, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBS2HA3V66
Biosample Biosource 
  1. Adenoma from mouse SA3961 - 4DNSR97J5Y12
  2. Adenoma from mouse SA3506 - 4DNSR4C1NJ87
  3. Adenoma from mouse SA3441 - 4DNSR5NR3PHL
  4. Adenoma from mouse SA3962 - 4DNSRA4LD892
Biosample Modifications 
Viral Transduction and Viral Transduction
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Yellow-green fiducial beads for drift correction
ch01
0
Chromosomes targeted by DAPI
ch02
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by Alexa 647-labelled imaging oligos
ch03
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by ATTO 565-labelled imaging oligos

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