ExperimentMic multiplexed FISH on whole fruit fly

multiplexed FISH4DNEXKDYHIQN
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Multiplexed FISH (Hi-M) of the dpp locus in intact drosophila embryos at nuclear cycle 12 (nc12), Biological Replicate 1, Technical Replicate 1

   September 10th, 2024 at 2:52pm
Source Publication
3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription.
Messina O, Raynal F, et al., Nature communications 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBS8FZSPGI
Biosample Biosource 
whole fruit fly - 4DNSRYC4ICIN
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Nuclei labelled by DAPI (first acquisition)
ch01
0
Fiducial mark acquired using Atto 550 (first acquisition)
ch02
0
Hybridization of imaging oligo to the reverse primer of the oligopaint library labelled with alexa flour 647 (first acquisition: mask0)
ch00
1-34
34 sequential ~12-kb loci in the dpp loci (fruit fly) targeted by oligo probe library (with atto-550-labelled fiducial oligo probes)
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