ExperimentMic Immunofluorescence on HeLa cell line with NCAPH insertion

Browser Suggestion

Google Chrome  or Mozilla Firefox are the recommended browser(s) for using the 4DN Data Portal.

Microsoft Edge, Safari, Opera etc. should work for a majority of portal functions but are not explicitly supported and may present some glitches, e.g. during submission.

Immunofluorescence4DNEXHWGZ2YB
released
View JSON
   September 13th, 2019 at 10:03pm
Source Publication
A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.
Abramo K, Valton AL, et al., Nature cell biology 2019

Properties  

Experiment Type 
Immunofluorescence
Follows SOP 
No
Biosample 
4DNBSUEJX8RU
Biosample Biosource 
HeLa cell line with NCAPH insertion - 4DNSRLVQY1PE
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
LaminA/C protein targeted by Alexa Fluor 488-labeled Lamin A Antibody (ab26300)
ch02
NCAPH protein targeted by dTomato-labeled NCAPH
ch03
TUBA4A protein targeted by Alexa Fluor 647-labeled Tubulin Antibody (SigmaT6199)

Experiment SetOpen In Search View