ExperimentMic Immunofluorescence on U2OS (Tier 2)

Immunofluorescence4DNEXHV5ZRHE
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Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on relatively thin targets. This sample targets one structure (mitochondrial nucleoids) which is expected to be inside of the organelle targeted by the other probe (mitochondrial outer membrane).

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Properties  

Experiment Type 
Immunofluorescence
Follows SOP 
No
Biosample 
4DNBSA3NSYOL
Biosample Biosource 
U2OS (Tier 2) - 4DNSR3V9ST6H
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
STORM
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
TOMM20 protein targeted by anti-TOMM20 (with anti-Rabbit, Alexa Fluor 647)
642nm
ch01
Chromosomes targeted by anti-ds DNA (with anti-Mouse, CF568)
560nm

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