ExperimentMic multiplexed FISH on A549

multiplexed FISH4DNEXHRMFIX1
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Multiplexed FISH of chromosome 22 in A549-Cas9 cancer cells from 3 coverslips where cells with CHD7 knocked-down using CHD7 targeting siRNA, Biological Replicate 1, Technical Replicate 1

   March 28th, 2025 at 1:37pm
Source Publication
Perturb-tracing enables high-content screening of multiscale 3D genome regulators.
Cheng Y, Hu M, et al., bioRxiv : the preprint server for biology 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSAZ2R6DM
Biosample Biosource 
A549 - 4DNSRFLY6T15
Biosample Modifications 
Viral Transduction and Viral Transduction deletion for CHD7 gene
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Chromosomes targeted by DAPI
ch01
1-14
Yellow-green fiducial beads for drift correction on chromosome 22
ch02
0
G1 phase detection
ch03
1-14
27 TADs imaged on Chr22 imaged using ATTO 565 imaging probes
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