ExperimentMic multiplexed FISH on ES-E14TG2a

multiplexed FISH4DNEXFU8K95M
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RNA FISH for nascent transcription of genes combined with ORCA labeling for 44 SEs, Biological replicate 1, Technical replicate 1

   May 21st, 2024 at 5:52pm
Source Publication
Super-enhancer interactomes from single cells link clustering and transcription.
Le DJ, Hafner A, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBS9ER6TE2
Biosample Biosource 
ES-E14TG2a - 4DNSRQDR15QF
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
Stanford-Boettiger_lab_ORCA_Nikon_Eclipse_Ti-U_Scope1
Channel
Imaging Rounds
Path
 
ch00
1-188
ORCA labeling of 188 SEs imaged using Alexa 647
ch01
1-188
ORCA labeling of 188 SEs imaged using Alexa 750
ch02
-
Image registration and drift correction using Cy3
ch03
-
ORCA labeling of 44 SEs combined with RNA-FISH

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