ExperimentMic multiplexed FISH on R1 mESC line

multiplexed FISH4DNEXEZBQPKD
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Multiplexed FISH in R1 mESC of the Prdm8-Fgf5 locus using CARGO-VPR system where CARGO arrays are used to recruit dCas9-VPR at site 33kb upstream of the Fgf5 promoter, Biological replicate 1, Technical replicate 1

   September 20th, 2024 at 3:24pm
Source Publication
Long range regulation of transcription scales with genomic distance in a gene specific manner.
Jensen CL, Chen LF, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSC38PJ56
Biosample Biosource 
R1 mESC line - 4DNSRKWHHO1Z
Biosample Modifications 
Stable Transfection insertion for Fgf5 mouse region
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
Stanford-Boettiger_lab_ORCA_Nikon_Eclipse_Ti-U_Scope1
Channel
Path
 
ch00
40 5kb-regions with 5kb intervals at Prdm8-Fgf5 locus (mouse) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
40 5kb-regions with 5kb intervals at Prdm8-Fgf5 locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes) for image registration and drift correction

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