ExperimentMic RNA FISH on G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion

RNA FISH4DNEXEVGGDL5
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RNA FISH of Hbb-b1, Tal1, and Slc4a1 intronic regions on 24h differentiated cells treated with 60min 1000nM flavopiridol. Note: no processed file present for Hbb-b1 (A594 channel) because no ROIs/transcription sites were identified.

   September 14th, 2018 at 4:51pm
Source Publication
Transcriptional Burst Initiation and Polymerase Pause Release Are Key Control Points of Transcriptional Regulation.
Bartman CR, Hamagami N, et al., Molecular cell 2018

Properties  

Experiment Type 
RNA FISH
Follows SOP 
No
Biosample 
4DNBSB6KAFDF
Biosample Biosource 
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion - 4DNSR41SLMH6
Biosample Modifications 
None
Biosample Treatments 
estradiol treatment (100 nM, 24h) and flavopiridol treatment (1000.0 nM, 60.0m)
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
GRCm38:7:103826534-103827928 mouse region targeted by Alexa Fluor 594-labeled custom oligos
ch02
GRCm38:4:115056426-115071755 mouse region targeted by Cy5-labeled custom oligos
ch03
GRCm38:11:102348820-102365281 mouse region targeted by Cy3-labeled custom oligos

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